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GPLD1 antibody

Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227
Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227
Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227
Anti-GPLD1 antibody used in Western Blot (WB). GTX33227
Anti-GPLD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33227

Cat. No. GTX33227

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GPLD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycosylphosphatidylinositol specific phospholipase D1 , GPIPLD , GPIPLDM , PIGPLD , PIGPLD1 , PLD

Cellular Localization

Secreted

Background

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227

GTX33227 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33227 GPLD1 antibody.
Dilution : 1:100

Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227

GTX33227 IHC-P Image

IHC-P analysis of human prostate tissue using GTX33227 GPLD1 antibody.
Dilution : 1:100

Anti-GPLD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33227

GTX33227 IHC-P Image

IHC-P analysis of rat heart tissue using GTX33227 GPLD1 antibody.
Dilution : 1:100

Anti-GPLD1 antibody used in Western Blot (WB). GTX33227

GTX33227 WB Image

WB analysis of various sample lysates using GTX33227 GPLD1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GPLD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33227

GTX33227 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33227 GPLD1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399