APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against mouse GPM6A Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of mouse GPM6A. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glycoprotein m6a , Gpm6 , M6A
Cellular Localization
Cell membrane,Cell projection, axon,Cell projection, dendritic spine,Cell projection, filopodium
Background
Predicted to enable calcium channel activity. Involved in neural retina development and neuron projection morphogenesis. Acts upstream of or within response to bacterium. Located in axonal growth cone. Is expressed in central nervous system; early nephron; intraembryonic coelom; sensory organ; and upper lip. Orthologous to human GPM6A (glycoprotein M6A). [provided by Alliance of Genome Resources, Apr 2022]
Database
Research Area
DATA IMAGES
![Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822](/templates/default/js/lightbox/images/loading.gif "Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822")
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GTX638822 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPM6A antibody [HL2473] (GTX638822) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638822 ICC/IF Image
GPM6A antibody [HL2473] detects GPM6A protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPM6A stained by GPM6A antibody [HL2473] (GTX638822) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638822 IHC-P Image
GPM6A antibody [HL2473] detects GPM6A protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GPM6A stained by GPM6A antibody [HL2473] (GTX638822) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638822 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPM6A antibody [HL2473] (GTX638822) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for GPM6A antibody [HL2473] (GTX638822). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPM6A antibody [HL2473] (GTX638822). Be the first to share your experience with this product.
![Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822](/upload/website/prouct_img/small/GTX638822/GTX638822_T-45096_20240126_WB_M_tissue_24013018_937.webp "Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822")
![Anti-GPM6A antibody [HL2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638822](/upload/website/prouct_img/small/GTX638822/GTX638822_T-45096_20231222_ICC_IF_R_24021917_496.webp "Anti-GPM6A antibody [HL2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638822")
![Anti-GPM6A antibody [HL2473] used in IHC (Paraffin sections) (IHC-P). GTX638822](/upload/website/prouct_img/small/GTX638822/GTX638822_T-45096_20240125_IHC-P_M_24021917_787.webp "Anti-GPM6A antibody [HL2473] used in IHC (Paraffin sections) (IHC-P). GTX638822")
![Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822](/upload/website/prouct_img/small/GTX638822/GTX638822_45369_20240412_WB_M_R_24041523_401.webp "Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822")
![Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822](/upload/website/prouct_img/normal/GTX638822/GTX638822_T-45096_20240126_WB_M_tissue_24013018_937.webp "Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822")
![Anti-GPM6A antibody [HL2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638822](/upload/website/prouct_img/normal/GTX638822/GTX638822_T-45096_20231222_ICC_IF_R_24021917_496.webp "Anti-GPM6A antibody [HL2473] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638822")
![Anti-GPM6A antibody [HL2473] used in IHC (Paraffin sections) (IHC-P). GTX638822](/upload/website/prouct_img/normal/GTX638822/GTX638822_T-45096_20240125_IHC-P_M_24021917_787.webp "Anti-GPM6A antibody [HL2473] used in IHC (Paraffin sections) (IHC-P). GTX638822")
![Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822](/upload/website/prouct_img/normal/GTX638822/GTX638822_45369_20240412_WB_M_R_24041523_401.webp "Anti-GPM6A antibody [HL2473] used in Western Blot (WB). GTX638822")