APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human GPNMB (AA: 31-260) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
glycoprotein nmb , HGFIN , NMB , PLCA3
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790](/templates/default/js/lightbox/images/loading.gif "Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790")
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GTX60790 ELISA Image
ELISA analysis of antigen using GTX60790 GPNMB antibody [7C10E5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60790 IHC-P Image
IHC-P analysis of breast cancer tissue using GTX60790 GPNMB antibody [7C10E5].
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GTX60790 WB Image
WB analysis of PANC1 (1) and PC-3 (2) cell lysate using GTX60790 GPNMB antibody [7C10E5].
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GTX60790 WB Image
WB analysis of HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate using GTX60790 GPNMB antibody [7C10E5].
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GTX60790 IHC-P Image
IHC-P analysis of esophagus cancer tissue using GTX60790 GPNMB antibody [7C10E5].
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REFERENCE
There are currently no references for GPNMB antibody [7C10E5] (GTX60790). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPNMB antibody [7C10E5] (GTX60790). Be the first to share your experience with this product.
![Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790](/upload/website/prouct_img/small/GTX60790/GTX60790_20170912_ELISA_18121411_766.jpg "Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790](/upload/website/prouct_img/small/GTX60790/GTX60790_20170912_IHC-P_18121411_479.jpg "Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790](/upload/website/prouct_img/small/GTX60790/GTX60790_20170912_WB_1_18121411_392.jpg "Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790](/upload/website/prouct_img/small/GTX60790/GTX60790_20170912_WB_18121411_460.jpg "Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790](/upload/website/prouct_img/small/GTX60790/GTX60790_20170912_IHC-P_1_18121411_436.jpg "Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790](/upload/website/prouct_img/normal/GTX60790/GTX60790_20170912_ELISA_18121411_766.jpg "Anti-GPNMB antibody [7C10E5] used in ELISA (ELISA). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790](/upload/website/prouct_img/normal/GTX60790/GTX60790_20170912_IHC-P_18121411_479.jpg "Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790](/upload/website/prouct_img/normal/GTX60790/GTX60790_20170912_WB_1_18121411_392.jpg "Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790](/upload/website/prouct_img/normal/GTX60790/GTX60790_20170912_WB_18121411_460.jpg "Anti-GPNMB antibody [7C10E5] used in Western Blot (WB). GTX60790")
![Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790](/upload/website/prouct_img/normal/GTX60790/GTX60790_20170912_IHC-P_1_18121411_436.jpg "Anti-GPNMB antibody [7C10E5] used in IHC (Paraffin sections) (IHC-P). GTX60790")