GeneTex
United States (US)

GPR120 antibody

GTX100364_43173_20180403_IHC-P_R_19011506_768.jpg
GTX100364_43173_20180420_WB_B_19011506_781.jpg
GTX100364_43173_20180403_IHC-P_M_19011506_652.jpg

Cat No. GTX100364

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

unboiled DDDDK-tagged GPR120-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GPR120. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BMIQ10 antibody , FFAR4 antibody , GPR129 antibody , GT01 antibody , O3FAR1 antibody , PGR4 antibody , free fatty acid receptor 4 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

GPR120 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).[supplied by OMIM]
DATA IMAGES
GTX100364_43173_20180403_IHC-P_R_19011506_768.jpg

GTX100364 IHC-P Image

GPR120 antibody [N1N2], N-term detects GPR120 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GPR120 stained by GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100364_43173_20180420_WB_B_19011506_781.jpg

GTX100364 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100364_43173_20180403_IHC-P_M_19011506_652.jpg

GTX100364 IHC-P Image

GPR120 antibody [N1N2], N-term detects GPR120 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GPR120 stained by GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169