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GPR120 antibody

Anti-GPR120 antibody used in Western Blot (WB). GTX100364
Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364
Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364
Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364

Cat. No. GTX100364

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399),
25 μl ($169)
See all GPR120 products
PRODUCT

Summary

GPR120 antibody detects G-protein-coupled receptor 120 (GPR120/FFAR4) (predicted molecular weight of 40 kDa), a GPCR activated by long-chain fatty acids such as omega-3 fatty acid. Stimulation of GPR120 with omega-3 elicits anti-inflammatory effects and enhances insulin sensitivity in a mouse model. In addition to its anti-inflammatory and metabolic functions, GPR120 may also impact immune function.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

unboiled DDDDK-tagged GPR120-transfected 293T

Predict Reactivity

Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR120. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240849

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

free fatty acid receptor 4 , BMIQ10 , GPR120 , GPR129 , GT01 , O3FAR1 , PGR4

Cellular Localization

Cell membrane

Background

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-GPR120 antibody used in Western Blot (WB). GTX100364

GTX100364 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364

GTX100364 IHC-P Image

GPR120 antibody [N1N2], N-term detects GPR120 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GPR120 stained by GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364

GTX100364 IHC-P Image

GPR120 antibody [N1N2], N-term detects GPR120 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GPR120 stained by GPR120 antibody [N1N2], N-term (GTX100364) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPR120 antibody used in IHC (Paraffin sections) (IHC-P). GTX100364

GTX100364 IHC-P Image

The data was published in the journal Front Physiol in 2019. PMID: 31214048

REFERENCE

Application Reference

Yokoji-Takeuchi M et al. Arch Oral Biol 2019; 110 : 104602 A bacterial metabolite induces Nrf2-mediated anti-oxidative responses in gingival epithelial cells by activating the MAPK signaling pathway.
Application : ICC/IF
Reactivity : Human
Tsai CC et al. Front Physiol 2019; 10 : 676 Long-Chain Fatty Acid Receptors Mediate Relaxation of the Porcine Lower Esophageal Sphincter.
Application : IHC-P
Reactivity : Pig
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169