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GPR125 antibody, Internal

Anti-GPR125 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88735
Anti-GPR125 antibody, Internal used in Western Blot (WB). GTX88735

Cat. No. GTX88735

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all GPR125 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

146 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88735-PEP).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor A3 , GPR125 , PGR21 , TEM5L

Cellular Localization

Membrane

Background

This gene encodes a member of the G protein-coupled receptor superfamily. This membrane protein may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES

Anti-GPR125 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88735

GTX88735 IHC-P Image

IHC-P analysis of human cortex using GTX88735 GPR125 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-GPR125 antibody, Internal used in Western Blot (WB). GTX88735

GTX88735 WB Image

WB analysis of human ovary lysate using GTX88735 GPR125 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Sakurai T et al. J Biol Chem 2022; 298 (10) : 102475 GPR125 (ADGRA3) is an autocleavable adhesion GPCR that traffics with Dlg1 to the basolateral membrane and regulates epithelial apicobasal polarity.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399