APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 6 μg/ml |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Gibbon, Gorilla, Marmoset(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic 16 amino acid peptide from C-terminus of human GPR149.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
RRID
AB_371294
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
G protein-coupled receptor 149 , G proteincoupled receptor 149 , GPR149 , IEDA , PGR10 , Pgr10
Cellular Localization
Cell membrane
Background
This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]
Database
Research Area
DATA IMAGES
|
GTX71579 IHC-P Image
IHC-P analysis of human testis, spermatocytic precursors tissue using GTX71579 GPR149 antibody.
Antigen retrieval : Heat-induced antigen retrieval
|
REFERENCE
There are currently no references for GPR149 antibody (GTX71579). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPR149 antibody (GTX71579). Be the first to share your experience with this product.
