GeneTex
United States (US)

GPR30 antibody

GTX100001_39224_WB_B_19011506_484.jpg

Cat No. GTX100001

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , GFP-human GPR30-transfected 293T cells (No boiling) , GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR30. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G Protein-Coupled Estrogen Receptor 1,Cepr,Cmkrl2,Dry12,Feg-1,Gpcr-Br,Gper,Gpr30,Lergu,Lergu2,Lygpr,Mer,Gper1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein

Background

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100001_39224_WB_B_19011506_484.jpg

GTX100001 WB Image

GPR30 antibody detects GPR30 protein by western blot analysis.
A. 50 µg 293T whole cell lysate/extract
B. 50 µg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling)
C. 50 µg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling)
5% SDS-PAGE
GPR30 antibody (GTX100001) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Due to the high tendency to aggregate, GPR30 samples were not boiled in the SDS gel-loading buffer before electrophoresis.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169