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GPR30 antibody

KO/KD
Anti-GPR30 antibody used in Western Blot (WB). GTX100001

Cat. No. GTX100001

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Pig
Package
100 μl ($399),
25 μl ($169)
See all GPR30 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

GFP-human GPR30-transfected 293T cells (No boiling)

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR30. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950438

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled estrogen receptor 1 , CEPR , CMKRL2 , DRY12 , FEG-1 , GPCR-Br , GPER , GPR30 , LERGU , LERGU2 , LyGPR , mER

Cellular Localization

Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein

Background

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPR30 antibody used in Western Blot (WB). GTX100001

GTX100001 WB Image

KO/KD

GPR30 antibody detects GPR30 protein by western blot analysis.
A. 50 μg 293T whole cell lysate/extract
B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling)
C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling)
5% SDS-PAGE
GPR30 antibody (GTX100001) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Due to the high tendency to aggregate, GPR30 samples were not boiled in the SDS gel-loading buffer before electrophoresis.

REFERENCE

Application Reference

LN Hoang et al. Cell Signal 2023; : 110733 Casein kinase 1 controls the shuttling of epidermal growth factor receptor and estrogen receptor in endometrial carcinoma induced by breast cancer hormonal therapy: Relevance of GPER1/Src
Reactivity : Human
Tran QK et al. J Biol Chem 2016; 291 (20) : 10805-23 Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.
Application : WB
Reactivity : Pig
Manuela Zavatti et al. Life Sciences 2014; Critical-size bone defect repair using amniotic fluid stem cell/collagen constructs: Effect of oral ferutinin treatment in rats
Application : IHC
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169