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GPR32 antibody [N1], N-term

Anti-GPR32 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108119
Anti-GPR32 antibody [N1], N-term used in Western Blot (WB). GTX108119

Cat No. GTX108119

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, Functional Assay

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all GPR32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

H1299

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GPR32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G protein-coupled receptor 32 , RVDR1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Orphan receptor.

Database

Research Area

DATA IMAGES

Anti-GPR32 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108119

GTX108119 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using GPR32(GTX108119) antibody at 1:500 dilution.

Anti-GPR32 antibody [N1], N-term used in Western Blot (WB). GTX108119

GTX108119 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
10% SDS PAGE
GTX108119 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Werz O et al. Nat Commun 2018; 9 (1) : 59 Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity.
Application : FACS
Reactivity : Human
Chatterjee A et al. FASEB J 2017; (Epub) Biosynthesis of proresolving lipid mediators by vascular cells and tissues.
Mottola G et al. PLoS One 2017; 12 (3) : e0174936 Aspirin-triggered resolvin D1 attenuates PDGF-induced vascular smooth muscle cell migration via the cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) pathway.
Application : Functional Assay
Orr SK et al. Am J Physiol Lung Cell Mol Physiol 2015; 308 (9) : L904-11 Proresolving actions of a new resolvin D1 analog mimetic qualifies as an immunoresolvent.
Application : FACS
Reactivity : Human
Dalli J et al. Chem Biol 2013; 20 (2) : 188-201 Resolvin D3 and aspirin-triggered resolvin D3 are potent immunoresolvents.
Application : FACS, Functional Assay
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169