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GPR32 antibody [N1], N-term

Anti-GPR32 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108119
Anti-GPR32 antibody [N1], N-term used in Western Blot (WB). GTX108119

Cat No. GTX108119

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, Functional Assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

H1299

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GPR32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G protein-coupled receptor 32 , RVDR1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Orphan receptor.

Database

Research Area

DATA IMAGES

Anti-GPR32 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108119

GTX108119 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using GPR32(GTX108119) antibody at 1:500 dilution.

Anti-GPR32 antibody [N1], N-term used in Western Blot (WB). GTX108119

GTX108119 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
10% SDS PAGE
GTX108119 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169