GeneTex
United States (US)

GPR40 antibody [C2C3], C-term

GTX100290_39428_IHC_19011506_383.jpg
GTX100290_WB_19011506_741.jpg

Cat No. GTX100290

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Free Fatty Acid Receptor 1,Ffa1R,Gpcr40,Gpr40,Ffar1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq]

Database

DATA IMAGES
GTX100290_39428_IHC_19011506_383.jpg

GTX100290 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human uterus, myometrium, using GPR40(GTX100290) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100290_WB_19011506_741.jpg

GTX100290 WB Image

Sample(30 µg of whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
15% SDS PAGE
GTX100290 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169