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GPR44 antibody

Anti-GPR44 antibody used in Western Blot (WB). GTX70387

Cat No. GTX70387

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($289)

APPLICATION

Application Note

Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

Reacts with residues 329-348 [DDSELGGAGSSRRRRTSSTA] of the CRTH2 (GPR44) protein. This sequence is near the C terminus. Cross reacts with rat and mouse.

PROPERTIES

Form

Liquid

Buffer

PBS containing 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Affinity purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Prostaglandin D2 Receptor 2 , Cd294 , Crth2 , Dl1R , Dp2 , Gpr44 , Ptgdr2

Background

CRTH2 (chemoattractant receptor-homologous molecule), which is also known as the putative G protein-coupled receptor (GPR44), is expressed on Th2 cells and is a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions.

Database

Research Area

DATA IMAGES

Anti-GPR44 antibody used in Western Blot (WB). GTX70387

GTX70387 WB Image

Western blot analysis of CRTH2 in CRTH2-transfected Jurkat cell lysate. Does not detect CRTH2 in non-transfected parental Jurkat cells.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf