GeneTex
United States (US)

GPR52 antibody

GTX108123_39757_20170518_WB_M_brain_19011507_939.jpg
GTX108123_39757_20170525_WB_Fraction_19011507_849.jpg
GTX108123_39757_CT_IHC_19011507_437.jpg

Cat No. GTX108123

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse brain , unboiled A549 , unboiled A549 membrane extract
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GPR52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G Protein-Coupled Receptor 52,Gpr52

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Members of the G protein-coupled receptor (GPR) family play important roles in signal transduction from the external environment to the inside of the cell.[supplied by OMIM]

Database

DATA IMAGES
GTX108123_39757_20170518_WB_M_brain_19011507_939.jpg

GTX108123 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPR52 antibody (GTX108123) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108123_39757_20170525_WB_Fraction_19011507_849.jpg

GTX108123 WB Image

Unboiled A549 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPR52 antibody (GTX108123) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108123_39757_CT_IHC_19011507_437.jpg

GTX108123 IHC-P Image

GPR52 antibody detects GPR52 protein at membrane on human small intestine by immunohistochemical analysis.
Sample: Paraffin-embedded small intestine.
GPR52 antibody (GTX108123) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169