GeneTex

  • Country / Location Selection

United States (US)

GPR52 antibody

KO/KD Orthogonal
Anti-GPR52 antibody used in Western Blot (WB). GTX108123
Anti-GPR52 antibody used in IHC (Paraffin sections) (IHC-P). GTX108123
Anti-GPR52 antibody used in Western Blot (WB). GTX108123
Anti-GPR52 antibody used in Western Blot (WB). GTX108123
Anti-GPR52 antibody used in Western Blot (WB). GTX108123

Cat. No. GTX108123

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GPR52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse brain , unboiled A549 , unboiled A549 membrane extract

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor 52

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Members of the G protein-coupled receptor (GPR) family play important roles in signal transduction from the external environment to the inside of the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GPR52 antibody used in Western Blot (WB). GTX108123

GTX108123 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPR52 antibody (GTX108123) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPR52 antibody used in IHC (Paraffin sections) (IHC-P). GTX108123

GTX108123 IHC-P Image

GPR52 antibody detects GPR52 protein at membrane on human small intestine by immunohistochemical analysis.
Sample: Paraffin-embedded small intestine.
GPR52 antibody (GTX108123) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPR52 antibody used in Western Blot (WB). GTX108123

GTX108123 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPR52 antibody (GTX108123) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPR52 antibody used in Western Blot (WB). GTX108123

GTX108123 WB Image

Orthogonal

Unboiled A549 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPR52 antibody (GTX108123) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPR52 antibody used in Western Blot (WB). GTX108123

GTX108123 WB Image

KO/KD

The data was published in the journal Elife in 2015. PMID: 25738228

REFERENCE

Application Reference

Song H et al. Brain 2018; 141 (6) : 1782-1798 Targeting Gpr52 lowers mutant HTT levels and rescues Huntington's disease-associated phenotypes.
Application : IHC-P
Reactivity : Human
Yao Y et al. Elife 2015; 4 A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for GPR52 antibody (GTX108123). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169