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GPR54 antibody

Anti-GPR54 antibody used in Western Blot (WB). GTX100374
Anti-GPR54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100374
Anti-GPR54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100374

Cat. No. GTX100374

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GPR54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GPR54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240861

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KISS1 receptor , AXOR12 , CPPB1 , GPR54 , HH8 , HOT7T175 , KISS-1R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPR54 antibody used in Western Blot (WB). GTX100374

GTX100374 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
C: Hep G2 (GTX27900)
10% SDS PAGE
GTX100374 diluted at 1:1000

Anti-GPR54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100374

GTX100374 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 24923321

Anti-GPR54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100374

GTX100374 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 24923321

REFERENCE

Application Reference

Mattam U et al. Exp Ther Med 2021; 22 (3) : 1019 Aging reduces kisspeptin receptor (GPR54) expression levels in the hypothalamus and extra-hypothalamic brain regions.
Application : WB
Reactivity : Rat
Sathagopam S. et al. Journal of Animal Reproduction and Biotechnology 2021; 36 (1) : 25-34 Chronic kisspeptin delays puberty and reduces feed intake and body weight in female rats.
Application : WB, IHC-P
Francis VA et al. PLoS One 2014; 9 (6) : e99680 Kisspeptin regulation of genes involved in cell invasion and angiogenesis in first trimester human trophoblast cells.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169