GPR82 antibody [N2C1], Internal

Cat. No. GTX100196

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all GPR82 products

Datasheet
Document

View product citations for antibody GTX100196 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GPR82. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037013

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor 82

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM]

Database

Gene ID: 27197 GPR82
UniProt: Q96P67 GPR82

DATA IMAGES

GTX100196 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100196 diluted at 1:1000

GTX100196 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GPR82(GTX100196) antibody at 1:200 dilution.

GTX100196 IHC-P Image

GPR82 antibody [N2C1], Internal detects GPR82 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: GPR82 antibody [N2C1], Internal (GTX100196) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Document
Unmodified.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty