GeneTex
United States (US)

GPR82 antibody [N2C1], Internal

GTX100196_39391_IFA_19011506_509.jpg
GTX100196_WB_19011506_399.jpg
GTX100196_39391_IHC_19011506_928.jpg

Cat No. GTX100196

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GPR82. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPR82 antibody, probable G-protein coupled receptor 82 antibody, G protein-coupled receptor 82 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.[supplied by OMIM]

Database

DATA IMAGES
GTX100196_39391_IFA_19011506_509.jpg

GTX100196 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GPR82(GTX100196) antibody at 1:200 dilution.

GTX100196_WB_19011506_399.jpg

GTX100196 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100196 diluted at 1:1000

GTX100196_39391_IHC_19011506_928.jpg

GTX100196 IHC-P Image

GPR82 antibody [N2C1], Internal detects GPR82 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: GPR82 antibody [N2C1], Internal (GTX100196) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169