GPRC5A antibody [HL1864]

GPRC5A antibody [HL1864] detects GPRC5A protein by immunofluorescent analysis.
Sample: Wild-type (WT) and GPRC5A knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920)

Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A  antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) unboiled MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A  antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A  antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A  antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GPRC5A antibody [HL1864] detects GPRC5A protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).