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GPS2 antibody [HL2276]

Recombinant antibody Orthogonal
Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329
Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329
Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329
Anti-GPS2 antibody [HL2276] used in IHC (Paraffin sections) (IHC-P). GTX638329
Anti-GPS2 antibody [HL2276] used in IHC (Paraffin sections) (IHC-P). GTX638329
Anti-GPS2 antibody [HL2276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638329
Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329

Cat. No. GTX638329

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2276

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GPS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human GPS2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein pathway suppressor 2 , AMF-1

Cellular Localization

Nucleus,Mitochondrion,Cytoplasm, cytosol

Background

This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329

GTX638329 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (GTX638329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329

GTX638329 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (GTX638329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329

GTX638329 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (GTX638329) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPS2 antibody [HL2276] used in IHC (Paraffin sections) (IHC-P). GTX638329

GTX638329 IHC-P Image

GPS2 antibody [HL2276] detects GPS2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse salivary gland.
GPS2 stained by GPS2 antibody [HL2276] (GTX638329) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPS2 antibody [HL2276] used in IHC (Paraffin sections) (IHC-P). GTX638329

GTX638329 IHC-P Image

GPS2 antibody [HL2276] detects GPS2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GPS2 stained by GPS2 antibody [HL2276] (GTX638329) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPS2 antibody [HL2276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638329

GTX638329 ICC/IF Image

GPS2 antibody [HL2276] detects GPS2 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in ice-cold MeOH for 5 min.
Green: GPS2 stained by GPS2 antibody [HL2276] (GTX638329) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GPS2 antibody [HL2276] used in Western Blot (WB). GTX638329

GTX638329 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (GTX638329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations