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GPS2 antibody

Orthogonal
Anti-GPS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117560
Anti-GPS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117560
Anti-GPS2 antibody used in Western Blot (WB). GTX117560

Cat. No. GTX117560

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GPS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173704

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein pathway suppressor 2 , AMF-1

Background

This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117560

GTX117560 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPS2(GTX117560) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117560

GTX117560 ICC/IF Image

GPS2 antibody detects GPS2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPS2 protein stained by GPS2 antibody (GTX117560) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-GPS2 antibody used in Western Blot (WB). GTX117560

GTX117560 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody (GTX117560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Zhuang Z et al. Am J Physiol Renal Physiol 2018; (Epub) G-protein Pathway Suppressor 2 (GPS2) enhanced the renal large conductance Ca2+ activated potassium channel expression via inhibiting ERK 1/2 signaling pathway.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169