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GPT antibody [N1N3]

Anti-GPT antibody [N1N3] used in Western Blot (WB). GTX101559
Anti-GPT antibody [N1N3] used in Western Blot (WB). GTX101559

Cat. No. GTX101559

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GPT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , HeLa , mouse liver

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GPT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240894

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic--pyruvic transaminase , AAT1 , ALT1 , GPT1

Cellular Localization

Cytoplasm

Background

Glutamate-pyruvate transaminase (EC 2.6.1.2), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GPT antibody [N1N3] used in Western Blot (WB). GTX101559

GTX101559 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GPT antibody [N1N3] (GTX101559) diluted at 1:500.

Anti-GPT antibody [N1N3] used in Western Blot (WB). GTX101559

GTX101559 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
10% SDS PAGE
GTX101559 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169