GeneTex
United States (US)

GPX2 antibody [C1C3]

KO/KD
GTX100292_39428_20160728_WB_shRNA_watermark_19011506_423.jpg
GTX100292_WB_19011506_471.jpg
GTX100292_39428_IHC_19011506_197.jpg
GTX100292_39428_IHC_M_19011506_361.jpg
GTX100292_39428_20150430_IP_19011506_851.jpg

Cat No. GTX100292

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutathione Peroxidase 2,Gi-Gpx,Gprp,Gprp-2,Gpx-2,Gpx-Gi,Gshpx-Gi,Gshpx-2,Gpx2

Cellular Localization

Cytoplasm

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100292_39428_20160728_WB_shRNA_watermark_19011506_423.jpg

GTX100292 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100292_WB_19011506_471.jpg

GTX100292 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX100292 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100292_39428_IHC_19011506_197.jpg

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100292_39428_IHC_M_19011506_361.jpg

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100292_39428_20150430_IP_19011506_851.jpg

GTX100292 IP Image

Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] (GTX100292).
Western blot analysis was performed using GPX2 antibody [C1C3] (GTX100292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169