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GPX2 antibody [C1C3]

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

Cat. No. GTX100292

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240895

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 2 , GI-GPx , GPRP , GPRP-2 , GPx-2 , GPx-GI , GSHPX-GI , GSHPx-2

Cellular Localization

Cytoplasm

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

GPX2 antibody detects GPX2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody (GTX100292) diluted by 1:500

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100292

GTX100292 ICC/IF Image

GPX2 antibody [C1C3] detects GPX2 protein at granule by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

GPX2 antibody [C1C3] detects GPX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody [C1C3] (GTX100292) diluted by 1:1000

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX100292

GTX100292 IP Image

Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] (GTX100292).
Western blot analysis was performed using GPX2 antibody [C1C3] (GTX100292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169