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GPX2 antibody [HL125]

Recombinant antibody KO/KD Orthogonal
Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477
Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477
Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477
Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477
Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477
Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477
Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477

Cat. No. GTX635477

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL125

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all GPX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888534

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 2 , GI-GPx , GPRP , GPRP-2 , GPx-2 , GPx-GI , GSHPX-GI , GSHPx-2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477

GTX635477 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477

GTX635477 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477

GTX635477 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477

GTX635477 IHC-P Image

GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [HL125] used in Western Blot (WB). GTX635477

GTX635477 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477

GTX635477 IHC-P Image

GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [HL125] used in IHC (Paraffin sections) (IHC-P). GTX635477

GTX635477 IHC-P Image

GPX2 antibody [HL125] detects GPX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations