APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888534
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutathione peroxidase 2 , GI-GPx , GPRP , GPRP-2 , GPx-2 , GPx-GI , GSHPX-GI , GSHPx-2
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
|
GTX635477 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX635477 IHC-P Image
GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX635477 WB Image
Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
GTX635477 IHC-P Image
GPX2 antibody [HL125] detects GPX2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX635477 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX635477 IHC-P Image
GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.GPX2 stained by GPX2 antibody [HL125] (GTX635477) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX635477 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [HL125] (GTX635477) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
REFERENCE
There are currently no references for GPX2 antibody [HL125] (GTX635477). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPX2 antibody [HL125] (GTX635477). Be the first to share your experience with this product.