APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.05% proclin300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GPX4,GPx4,GSHPx4,MCSP,PHGPx,SMDS,glutathione peroxidase 4,snGPx,snPHGPx,Glutathione Peroxidase 4
Cellular Localization
Mitochondrion,Cytoplasm
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. [provided by RefSeq, Dec 2018]
Database
Research Area
DATA IMAGES
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GTX04794 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX04794 GPX4 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:200
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GTX04794 IHC-P Image
IHC-P analysis of rat testis tissue using GTX04794 GPX4 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:200
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GTX04794 WB Image
WB analysis of Jurkat whole cell lysate using GTX04794 GPX4 antibody. Loading : 25 μg Dilution : 1:1000
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GTX04794 WB Image
WB analysis of U-87MG whole cell lysate with/without GPX4 knockdown treatment using GTX04794 GPX4 antibody. Loading : 25 μg Dilution : 1:1000
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GTX04794 WB Image
WB analysis of various samples using GTX04794 GPX4 antibody. Lane 1 : Mouse testis tissue lysate Lane 2 : Rat testis tissue lysate Loading : 25 μg Dilution : 1:1000
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REFERENCE
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REVIEW
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