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GPX7 antibody

KO/KD
Anti-GPX7 antibody used in IHC (Paraffin sections) (IHC-P). GTX108578
Anti-GPX7 antibody used in Western Blot (WB). GTX108578
Anti-GPX7 antibody used in Western Blot (WB). GTX108578

Cat. No. GTX108578

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GPX7 products
PRODUCT

Summary

GPX7 antibody detects glutathione peroxidase 7 (GPX7), a secreted enzyme with a predicted molecular weight of ~21 kDa. GPX7 serves as a stress sensor and regulates several genes involved in reactive oxygen species (ROS)-induced DNA damage.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent

Note :

WB
Recommend using PVDF membrane.
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Mouse embryonic fibroblast

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human GPX7

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037098

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 7 , CL683 , GPX6 , GPx-7 , GSHPx-7 , NPGPx

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-GPX7 antibody used in IHC (Paraffin sections) (IHC-P). GTX108578

GTX108578 IHC-P Image

GPX7 antibody detects GPX7 protein at cytoplasm on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
GPX7 antibody (GTX108578) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX7 antibody used in Western Blot (WB). GTX108578

GTX108578 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX7 antibody (GTX108578) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX7 antibody used in Western Blot (WB). GTX108578

GTX108578 WB Image

KO/KD

Sample (20 ug of whole cell lysate)

A: Mouse embryonic fibroblast (WT)

B: Mouse embryonic fibroblast(Gpx7 -/-)

12% SDS PAGE

GTX108578 diluted at 1:1000

REFERENCE

Application Reference

Ghatak S et al. Mol Ther 2023; 31 (2) : 454-470 Driving adult tissue repair via re-engagement of a pathway required for fetal healing.
Hsieh YL et al. Cell Rep 2019; 29 (8) : 2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Application : WB
Reactivity : Mouse
Wei PC et al. Mol Cell 2012; 48 (5) : 747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Application : IP
Reactivity : Mouse
Wei PC et al. Nucleic Acids Res 2012; 40 (1) : 323-32 Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Application : IP
Submit a Reference

REVIEW

GPX7 antibody Cat. No. GTX108578

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GPX7 antibody used in Western Blot (WB). GTX108578
Date : Anonymous submitted on 02-Jul-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Hs68 cells
Amount used : 40 μg
Blocking : 5% milk, 25°C, 0.25Hr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169