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GPX8 antibody

Orthogonal
Anti-GPX8 antibody used in Western Blot (WB). GTX125992
Anti-GPX8 antibody used in Western Blot (WB). GTX125992
Anti-GPX8 antibody used in Western Blot (WB). GTX125992
Anti-GPX8 antibody used in Western Blot (WB). GTX125992
Anti-GPX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125992
Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992
Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992
Anti-GPX8 antibody used in Western Blot (WB). GTX125992

Cat. No. GTX125992

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GPX8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPX8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885626

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 8 (putative) , EPLA847 , GPx-8 , GSHPx-8 , UNQ847

Cellular Localization

Membrane; Single-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX8 antibody (GTX125992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX8 antibody (GTX125992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX8 antibody (GTX125992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX8 antibody (GTX125992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125992

GTX125992 ICC/IF Image

GPX8 antibody detects GPX8 protein by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX8 stained by GPX8 antibody (GTX125992) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992

GTX125992 IHC-P Image

GPX8 antibody detects GPX8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
GPX8 antibody (GTX125992) dilution: 1:500.


Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992

GTX125992 IHC-P Image

GPX8 antibody detects GPX8 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GPX8 stained by GPX8 antibody (GTX125992) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

The data was published in the journal Aging Cell in 2018. PMID: 29659168

REFERENCE

Application Reference

Bai Y et al. BMC Med Genomics 2024; 17 (1) : 77 GPX8(+) cancer-associated fibroblast, as a cancer-promoting factor in lung adenocarcinoma, is related to the immunosuppressive microenvironment.
Fang J et al. Aging Cell 2018; 17 (4) : e12765
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169