GeneTex
United States (US)

GPX8 antibody

GTX125992_41122_WB_18121410_352.jpg
GTX125992_41122_IHC_18121410_830.jpg
GTX125992_43278_20190306_ICC_IF_19042202_737.jpg

Cat No. GTX125992

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

NT2D1 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPX8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutathione Peroxidase 8 (Putative),Epla847,Gpx-8,Gshpx-8,Unq847,Gpx8

Cellular Localization

Membrane; Single-pass membrane protein

Database

DATA IMAGES
GTX125992_41122_WB_18121410_352.jpg

GTX125992 WB Image

GPX8 antibody detects GPX8 protein by Western blot analysis.
A. 30 µg NT2D1 whole cell lysate/extract
B. 30 µg U87-MG whole cell lysate/extract
12 % SDS-PAGE
GPX8 antibody (GTX125992) dilution: 1:1000

GTX125992_41122_IHC_18121410_830.jpg

GTX125992 IHC-P Image

GPX8 antibody detects GPX8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
GPX8 antibody (GTX125992) dilution: 1:500.


Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX125992_43278_20190306_ICC_IF_19042202_737.jpg

GTX125992 ICC/IF Image

GPX8 antibody detects GPX8 protein by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX8 stained by GPX8 antibody (GTX125992) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169