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GPX8 antibody

Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992
Anti-GPX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125992
Anti-GPX8 antibody used in Western Blot (WB). GTX125992

Cat No. GTX125992

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

NT2D1 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPX8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885626

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutathione peroxidase 8 (putative) , EPLA847 , GPx-8 , GSHPx-8 , UNQ847

Cellular Localization

Membrane; Single-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-GPX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX125992

GTX125992 IHC-P Image

GPX8 antibody detects GPX8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
GPX8 antibody (GTX125992) dilution: 1:500.


Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125992

GTX125992 ICC/IF Image

GPX8 antibody detects GPX8 protein by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX8 stained by GPX8 antibody (GTX125992) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GPX8 antibody used in Western Blot (WB). GTX125992

GTX125992 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GPX8 antibody (GTX125992) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

GPX8 antibody Cat No. GTX125992

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Aging Cell in 2018.PMID: 29659168
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169