*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX15916-PEP).
This antibody is expected to recognise both reported isoforms.
Mouse, Rat, Bovine, Dog(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1; NP_001129080.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Rho Gtpase Activating Protein 26,Graf,Graf1,Ophn1L,Ophn1L1,Arhgap26
Colocalizes with actin stress fibers and cortical actin structures.
Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
GTX15916 WB Image
WB analysis of human heart lysate with (B) and without (A) blocking with the immunising peptide using GTX15916 GRAF antibody, C-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer
GTX15916 IHC-P Image
IHC-P analysis of human colon using GTX15916 GRAF antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml