GeneTex
United States (US)

GRAF antibody, C-term

GTX15916_1427_WB_18121410_107.jpg
GTX15916_406_IHC-P_18121410_803.jpg

Cat No. GTX15916

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15916-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both reported isoforms.

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1; NP_001129080.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Gtpase Activating Protein 26,Graf,Graf1,Ophn1L,Ophn1L1,Arhgap26

Cellular Localization

Colocalizes with actin stress fibers and cortical actin structures.

Background

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

DATA IMAGES
GTX15916_1427_WB_18121410_107.jpg

GTX15916 WB Image

WB analysis of human heart lysate with (B) and without (A) blocking with the immunising peptide using GTX15916 GRAF antibody, C-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX15916_406_IHC-P_18121410_803.jpg

GTX15916 IHC-P Image

IHC-P analysis of human colon using GTX15916 GRAF antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 329