APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX15916-PEP).
Product Note
This antibody is expected to recognise both reported isoforms.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1; NP_001129080.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho GTPase activating protein 26 , GRAF , GRAF1 , OPHN1L , OPHN1L1
Cellular Localization
Cytoplasm, cytoskeleton
Background
Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]
Database
Research Area
DATA IMAGES
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GTX15916 WB Image
WB analysis of human heart lysate with (B) and without (A) blocking with the immunising peptide using GTX15916 GRAF antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX15916 IHC-P Image
IHC-P analysis of human colon using GTX15916 GRAF antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for GRAF antibody, C-term (GTX15916). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GRAF antibody, C-term (GTX15916). Be the first to share your experience with this product.