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GRAF antibody [C2C3], C-term

Anti-GRAF antibody [C2C3], C-term used in Western Blot (WB). GTX117598

Cat No. GTX117598

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A549 , HepG2 , HCT116

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175723

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho GTPase activating protein 26 , GRAF , GRAF1 , OPHN1L , OPHN1L1

Cellular Localization

Cell junction , focal adhesion , Cytoplasm , cytoskeleton

Background

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRAF antibody [C2C3], C-term used in Western Blot (WB). GTX117598

GTX117598 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX117598 diluted at 1:1000

REFERENCE

There are currently no references for GRAF antibody [C2C3], C-term (GTX117598). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GRAF antibody [C2C3], C-term (GTX117598). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169