GeneTex
United States (US)

GRAP antibody [C2C3], C-term

GTX104623_39631_IHC_19011506_855.jpg
GTX104623_39631_IFA_19011506_349.jpg
GTX104623_WB_19011506_210.jpg

Cat No. GTX104623

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC64880 antibody, GRAP antibody, growth factor receptor-bound protein 2-related adaptor protein antibody, GRB2-related adapter protein antibody, GRB2-related adaptor protein antibody

Background

This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104623_39631_IHC_19011506_855.jpg

GTX104623 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using GRAP(GTX104623) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104623_39631_IFA_19011506_349.jpg

GTX104623 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using GRAP(GTX104623) antibody at 1:500 dilution.

GTX104623_WB_19011506_210.jpg

GTX104623 WB Image

Sample (30 ug of whole cell lysate)
A: A549 12% SDS PAGE
GTX104623 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169