GeneTex

  • Country / Location Selection

United States (US)

GRASP65 antibody [4F8]

Overexpression
Anti-GRASP65 antibody [4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84422
Anti-GRASP65 antibody [4F8] used in Western Blot (WB). GTX84422
Anti-GRASP65 antibody [4F8] used in Western Blot (WB). GTX84422

Cat. No. GTX84422

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F8

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($519)
See all GRASP65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi reassembly stacking protein 1 , GOLPH5 , GRASP65 , P65

Background

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.

Database

Research Area

DATA IMAGES

Anti-GRASP65 antibody [4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84422

GTX84422 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GRASP65 plasmid using GTX84422 GRASP65 antibody [4F8].

Anti-GRASP65 antibody [4F8] used in Western Blot (WB). GTX84422

GTX84422 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GRASP65 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84422 GRASP65 antibody [4F8].
Loading : 5 ug per lane

Anti-GRASP65 antibody [4F8] used in Western Blot (WB). GTX84422

GTX84422 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84422 GRASP65 antibody [4F8].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

There are currently no references for GRASP65 antibody [4F8] (GTX84422). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GRASP65 antibody [4F8] (GTX84422). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519