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GRASP65 antibody [5C5]

Overexpression
Anti-GRASP65 antibody [5C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84423
Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423
Anti-GRASP65 antibody [5C5] used in Western Blot (WB). GTX84423
Anti-GRASP65 antibody [5C5] used in Flow cytometry (FACS). GTX84423
Anti-GRASP65 antibody [5C5] used in Western Blot (WB). GTX84423
Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423
Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423

Cat. No. GTX84423

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C5

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Dog
Package
100 μl ($519)
See all GRASP65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:500
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi reassembly stacking protein 1 , GOLPH5 , GRASP65 , P65

Background

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.

Database

Research Area

DATA IMAGES

Anti-GRASP65 antibody [5C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84423

GTX84423 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GRASP65 plasmid using GTX84423 GRASP65 antibody [5C5].

Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423

GTX84423 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84423 GRASP65 antibody [5C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GRASP65 antibody [5C5] used in Western Blot (WB). GTX84423

GTX84423 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GRASP65 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84423 GRASP65 antibody [5C5].
Loading : 5 ug per lane

Anti-GRASP65 antibody [5C5] used in Flow cytometry (FACS). GTX84423

GTX84423 FACS Image

FACS analysis of HEK293T cells transfected with either GRASP65 plasmid(Red) or empty vector control plasmid(Blue) using GTX84423 GRASP65 antibody [5C5].

Anti-GRASP65 antibody [5C5] used in Western Blot (WB). GTX84423

GTX84423 WB Image

WB analysis of various cell lines using GTX84423 GRASP65 antibody [5C5].
Loading : 35 ug per lane

Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423

GTX84423 IHC-P Image

IHC-P analysis of human liver tissue using GTX84423 GRASP65 antibody [5C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GRASP65 antibody [5C5] used in IHC (Paraffin sections) (IHC-P). GTX84423

GTX84423 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84423 GRASP65 antibody [5C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519