GRASP65 antibody [5G8]

Cat. No. GTX84424

Host	Mouse
Clonality	Monoclonal
Clone Name	5G8
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84424 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi reassembly stacking protein 1 , GOLPH5 , GRASP65 , P65

Background

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.

Database

Gene ID: 64689 GORASP1
UniProt: Q9BQQ3 GORASP1

Research Area

DATA IMAGES

GTX84424 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84424 GRASP65 antibody [5G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84424 WB Image

WB analysis of various cell lines using GTX84424 GRASP65 antibody [5G8].
Loading : 35 ug per lane

GTX84424 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GRASP65 plasmid using GTX84424 GRASP65 antibody [5G8].

GTX84424 FACS Image

FACS analysis of HEK293T cells transfected with either GRASP65 plasmid(Red) or empty vector control plasmid(Blue) using GTX84424 GRASP65 antibody [5G8].

GTX84424 WB Image

WB analysis of HEK293T cells transfected with GRASP65 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84424 GRASP65 antibody [5G8].
Loading : 5 ug per lane

GTX84424 FACS Image

FACS analysis of Jurkat cells using GTX84424 GRASP65 antibody [5G8].
Red : Primary antibody
Blue : Negative control antibody

GTX84424 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84424 GRASP65 antibody [5G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84424 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84424 GRASP65 antibody [5G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84424 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84424 GRASP65 antibody [5G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

GRASP65 antibody [5G8]

Cat. No. GTX84424

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX84424 IHC-P Image

GTX84424 WB Image

GTX84424 ICC/IF Image

GTX84424 FACS Image

GTX84424 WB Image

GTX84424 FACS Image

GTX84424 IHC-P Image

GTX84424 IHC-P Image

GTX84424 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.