GeneTex
United States (US)

GRASP65 antibody

GTX32631_20180530_WB_18121410_660.jpg
GTX32631_20180530_ICCIF_18121410_491.jpg
GTX32631_20180530_IHC-P_18121410_181.jpg

Cat No. GTX32631

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Golgi Reassembly Stacking Protein 1,Golph5,Grasp65,P65,Gorasp1

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX32631_20180530_WB_18121410_660.jpg

GTX32631 WB Image

WB analysis of various samples using GTX32631 GRASP65 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32631_20180530_ICCIF_18121410_491.jpg

GTX32631 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32631 GRASP65 antibody.
Blue : DAPI

GTX32631_20180530_IHC-P_18121410_181.jpg

GTX32631 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX32631 GRASP65 antibody.
Dilution : 1:100

Package List Price ($)
$ 329