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GRASP65 antibody

Anti-GRASP65 antibody used in IHC (Paraffin sections) (IHC-P). GTX32631
Anti-GRASP65 antibody used in Western Blot (WB). GTX32631
Anti-GRASP65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32631

Cat. No. GTX32631

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all GRASP65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi reassembly stacking protein 1 , GOLPH5 , GRASP65 , P65

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-GRASP65 antibody used in IHC (Paraffin sections) (IHC-P). GTX32631

GTX32631 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX32631 GRASP65 antibody.
Dilution : 1:100

Anti-GRASP65 antibody used in Western Blot (WB). GTX32631

GTX32631 WB Image

WB analysis of various sample lysates using GTX32631 GRASP65 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GRASP65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32631

GTX32631 ICC/IF Image

ICC/IF analysis of A549 cells using GTX32631 GRASP65 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399