GeneTex
United States (US)

GRB10 (phospho Tyr67) antibody

GTX32187_20190131_ICCIF_19013101_359.jpg
GTX32187_20190131_WB_19013102_140.jpg

Cat No. GTX32187

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of GRB10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Growth Factor Receptor Bound Protein 10,Grb-Ir,Grb-10,Irbp,Meg1,Rss,Grb10

Cellular Localization

Cytoplasm

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES
GTX32187_20190131_ICCIF_19013101_359.jpg

GTX32187 ICC/IF Image

ICC/IF analysis of formalin-fixed PC12 cells using GTX32187 GRB10 (phospho Tyr67) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32187_20190131_WB_19013102_140.jpg

GTX32187 WB Image

WB analysis of insulin-treated HEK293T (A), mouse spleen (B), insulin-treated PC12 (C) whole cell lysates using GTX32187 GRB10 (phospho Tyr67) antibody.

Package List Price ($)
$ 319