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GRB14 antibody [N1C2]

Anti-GRB14 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108911
Anti-GRB14 antibody [N1C2] used in Western Blot (WB). GTX108911
Anti-GRB14 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108911
Anti-GRB14 antibody [N1C2] used in Western Blot (WB). GTX108911

Cat No. GTX108911

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($379),
25 μl ($169)
See all GRB14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRB14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950449

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth factor receptor bound protein 14

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Endosome membrane; Peripheral membrane protein

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date. [provided by RefSeq]

Database

DATA IMAGES

Anti-GRB14 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108911

GTX108911 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using GRB14(GTX108911) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB14 antibody [N1C2] used in Western Blot (WB). GTX108911

GTX108911 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108911 diluted at 1:1000

Anti-GRB14 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX108911

GTX108911 IHC-P Image

GRB14 antibody [N1C2] detects GRB14 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GRB14 antibody [N1C2] (GTX108911) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB14 antibody [N1C2] used in Western Blot (WB). GTX108911

GTX108911 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX108911 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169