Human tissue extract were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRB14 antibody [N1C2] (GTX108911) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GRB14 antibody [N1C2] detects GRB14 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GRB14 antibody [N1C2] (GTX108911) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108911 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using GRB14(GTX108911) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min