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GRB2 antibody

Anti-GRB2 antibody used in Western Blot (WB). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294
Anti-GRB2 antibody used in Western Blot (WB). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294
Anti-GRB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100294
Anti-GRB2 antibody used in Western Blot (WB). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

Cat No. GTX100294

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , BCL-1

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

growth factor receptor bound protein 2,ASH,EGFRBP-GRB2,Grb3-3,MST084,MSTP084,NCKAP2

Cellular Localization

Golgi apparatus

Background

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GRB2 antibody used in Western Blot (WB). GTX100294

GTX100294 WB Image

Wild-type (WT) and GRB2 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRB2 antibody (GTX100294) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB2 antibody used in Western Blot (WB). GTX100294

GTX100294 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
12% SDS PAGE
GTX100294 diluted at 1:1000

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100294

GTX100294 ICC/IF Image

GRB2 antibody detects GRB2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRB2 protein stained by GRB2 antibody (GTX100294) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-GRB2 antibody used in Western Blot (WB). GTX100294

GTX100294 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRB2 antibody (GTX100294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100294

GTX100294 IHC-P Image

GRB2 antibody detects GRB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169