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GRB2 antibody

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22234
Anti-GRB2 antibody used in Western Blot (WB). GTX22234
Anti-GRB2 antibody used in Western Blot (WB). GTX22234
Anti-GRB2 antibody used in Western Blot (WB). GTX22234

Cat. No. GTX22234

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Pig
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.05-0.15μg/ml
IHC-P 1-2μg/ml

Note :

IHC-P
Human Lymph Node shows nuclear staining in some cells inside the germinal centre.

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22234-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth factor receptor bound protein 2 , ASH , EGFRBP-GRB2 , Grb3-3 , MST084 , MSTP084 , NCKAP2

Cellular Localization

Nucleus , Cytoplasm , Endosome , Golgi apparatus

Background

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX22234

GTX22234 IHC-P Image

IHC-P analysis of human lymph node using GTX22234 GRB2 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 1μg/ml

Anti-GRB2 antibody used in Western Blot (WB). GTX22234

GTX22234 WB Image

WB analysis of human (A) and pig (B) spleen lysates using GTX22234 GRB2 antibody.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GRB2 antibody used in Western Blot (WB). GTX22234

GTX22234 WB Image

WB analysis of human thymus (A) and Molt-4 (B) lysates using GTX22234 GRB2 antibody.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GRB2 antibody used in Western Blot (WB). GTX22234

GTX22234 WB Image

WB analysis of mouse (A+C) and rat (B+D) brain (A+B) and spleen (C+D) lysates using GTX22234 GRB2 antibody.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for GRB2 antibody (GTX22234). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399