APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.15μg/ml |
| 1-2μg/ml |
|
Note :
IHC-P
Human Lymph Node shows nuclear staining in some cells inside the germinal centre.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX22234-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
growth factor receptor bound protein 2 , ASH , EGFRBP-GRB2 , Grb3-3 , MST084 , MSTP084 , NCKAP2
Cellular Localization
Nucleus , Cytoplasm , Endosome , Golgi apparatus
Background
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX22234 WB Image
WB analysis of human (A) and pig (B) spleen lysates using GTX22234 GRB2 antibody.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
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GTX22234 WB Image
WB analysis of human thymus (A) and Molt-4 (B) lysates using GTX22234 GRB2 antibody.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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GTX22234 IHC-P Image
IHC-P analysis of human lymph node using GTX22234 GRB2 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 1μg/ml
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GTX22234 WB Image
WB analysis of mouse (A+C) and rat (B+D) brain (A+B) and spleen (C+D) lysates using GTX22234 GRB2 antibody.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for GRB2 antibody (GTX22234). Be the first to share your publications with this product.
REVIEW
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