APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:10 - 1:100 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRB2 (NP_002077.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
growth factor receptor bound protein 2 , ASH , EGFRBP-GRB2 , Grb3-3 , MST084 , MSTP084 , NCKAP2
Cellular Localization
Nucleus,Cytoplasm,Endosome,Golgi apparatus
Background
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX32632 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX32632 GRB2 antibody. Blue : DAPI
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GTX32632 WB Image
WB analysis of various sample lysates using GTX32632 GRB2 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX32632 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32632 GRB2 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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