GeneTex
United States (US)

GREM2 antibody [C1C3]

GTX108414_39834_CT_IHC_19011507_445.jpg
GTX108414_39834_20160428_WB_2_19011507_428.jpg
GTX108414_39834_20160428_WB_19011507_726.jpg

Cat No. GTX108414

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

A549 , IMR32

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GREM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gremlin 2, Dan Family Bmp Antagonist,Cktsf1B2,Dand3,Prdc,Sthag9,Grem2

Cellular Localization

Secreted

Background

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. [provided by RefSeq]

Database

DATA IMAGES
GTX108414_39834_CT_IHC_19011507_445.jpg

GTX108414 IHC-P Image

GREM2 antibody [C1C3] detects GREM2 protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
GREM2 antibody [C1C3] (GTX108414) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108414_39834_20160428_WB_2_19011507_428.jpg

GTX108414 WB Image

Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with GREM2 antibody [C1C3] (GTX108414) diluted at 1:500.

GTX108414_39834_20160428_WB_19011507_726.jpg

GTX108414 WB Image

Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with GREM2 antibody [C1C3] (GTX108414) diluted at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169