GRF1 (phospho Tyr1105) antibody

Cat. No. GTX52344

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μg ($399)

See all GRF1 products

Datasheet
Document

View product citations for antibody GTX52344 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200

Not tested in other applications.

Calculated MW

171 kDa. ( Note )

Product Note

GRLF1

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GRF1 around the phosphorylation site of Tyr1105

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 35 , GRF-1 , GRLF1 , P190-A , P190A , p190ARhoGAP , p190RhoGAP

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2909 ARHGAP35
UniProt: Q9NRY4 ARHGAP35

Research Area

DATA IMAGES

GTX52344 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX52344 GRF1 (phospho Tyr1105) antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:200

GTX52344 ICC/IF Image

ICC/IF analysis of 293 cells using GTX52344 GRF1 (phospho Tyr1105) antibody.
GTX52344_IFAHC-P_20180601: IHC-P analysis of human lung cancer tissue using GTX52344 GRF1 (phospho Tyr1105) antibody.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)

GTX52344 WB Image

Western blot analysis of GRF-1 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty