APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88972-PEP).
Product Note
This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence according to NP_000821.1; NP_783300.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate ionotropic receptor kainate type subunit 1 , EAA3 , EEA3 , GLR5 , GLUR5 , GluK1 , gluR-5
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX88972 WB Image
WB analysis of human brain (cerebellum) lysate using GTX88972 GRIK1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX88972 IHC-P Image
IHC-P analysis of human cortex using GTX88972 GRIK1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
|
REFERENCE
There are currently no references for GRIK1 antibody, Internal (GTX88972). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GRIK1 antibody, Internal (GTX88972). Be the first to share your experience with this product.
