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GRIK1 antibody, Internal

Anti-GRIK1 antibody, Internal used in Western Blot (WB). GTX88972
Anti-GRIK1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88972

Cat No. GTX88972

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88972-PEP).

Product Note

This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence according to NP_000821.1; NP_783300.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamate ionotropic receptor kainate type subunit 1 , EAA3 , EEA3 , GLR5 , GLUR5 , GluK1 , gluR-5

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GRIK1 antibody, Internal used in Western Blot (WB). GTX88972

GTX88972 WB Image

WB analysis of human brain (cerebellum) lysate using GTX88972 GRIK1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GRIK1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88972

GTX88972 IHC-P Image

IHC-P analysis of human cortex using GTX88972 GRIK1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369