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GRK1 antibody [G8]

Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775
Anti-GRK1 antibody [G8] used in IHC (Frozen sections) (IHC-Fr). GTX22775
Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775
Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775
Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775

Cat. No. GTX22775

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 G8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Rat, Bovine, Dog, Chicken
Package
100 μg ($399)
See all GRK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 2-3 μg/ml
IHC-Fr Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Product Note

This antibody recognizes an epitope in the C-terminal region of GRK1a.

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GRK1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 1 , GPRK1 , RHOK , RK

Cellular Localization

Membrane

Background

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775

GTX22775 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22775 GRK1 antibody [G8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

Anti-GRK1 antibody [G8] used in IHC (Frozen sections) (IHC-Fr). GTX22775

GTX22775 IHC-Fr Image

IHC-Fr analysis of bovine retina tissue using GTX22775 GRK1 antibody [G8].

Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775

GTX22775 ICC/IF Image

ICC/IF analysis of murine cells using GTX22775 GRK1 antibody [G8].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775

GTX22775 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22775 GRK1 antibody [G8]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-GRK1 antibody [G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22775

GTX22775 ICC/IF Image

ICC/IF analysis of human cells using GTX22775 GRK1 antibody [G8].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight in a humidified chamber

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399