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GRK2 antibody [C2C3], C-term

KO/KD Overexpression
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682

Cat. No. GTX101682

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

ADRBK1-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

KO/KD

Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101682

GTX101682 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GTX101682) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682

GTX101682 ICC/IF Image

GRK2 antibody [C2C3], C-term detects GRK2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GRK2 stained by GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GRK2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101682

GTX101682 IP Image

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody [C2C3], C-term (GTX101682).
Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682

GTX101682 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GTX101682) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Anna Barkaway et al. Immunity 2021; 54 (7) : 1494-1510.e7 Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.
Application : WB
Reactivity : Mouse
Vincent Yi-Fong Su et al. Int J Mol Sci 2021; 22 (18) Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury.
Application : ICC/IF
Reactivity : Mouse
Huang P et al. Int Immunopharmacol 2016; 39 : 377-82 Astragaloside IV alleviates E. coli-caused peritonitis via upregulation of neutrophil influx to the site of infection.
Application : FACS
Dasu MR et al. Stem Cells Transl Med 2014; 3 (6) : 745-59 Crosstalk between adrenergic and toll-like receptors in human mesenchymal stem cells and keratinocytes: a recipe for impaired wound healing.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169