GeneTex
United States (US)

GRK2 antibody [C2C3], C-term

Overexpression
GTX101682_39995_20141128_WB_19011506_415.jpg
GTX101682_39995_20180831_WB_B_19011506_347.jpg
GTX101682_39995_IHC_19011506_617.jpg
GTX101682_WB_M_19011506_504.jpg
GTX101682_39995_IFA_19011506_359.jpg
GTX101682_WB_19011506_689.jpg
GTX101682_39995_20150522_IP_19011506_749.jpg

Cat No. GTX101682

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , Jurkat , NCI-H929

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BARK1 antibody, BETA-ARK1 antibody, FLJ16718 antibody, GRK2 antibody, ADRBK1 antibody, G-protein coupled receptor kinase 2 antibody, beta-ARK-1 antibody, beta-adrenergic receptor kinase 1 antibody, "adrenergic, beta, receptor kinase 1 antibody"

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX101682_39995_20141128_WB_19011506_415.jpg

GTX101682 WB Image

GRK2 antibody detects GRK2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX101682) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101682_39995_20180831_WB_B_19011506_347.jpg

GTX101682 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101682_39995_IHC_19011506_617.jpg

GTX101682 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GTX101682) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101682_WB_M_19011506_504.jpg

GTX101682 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101682 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101682_39995_IFA_19011506_359.jpg

GTX101682 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GTX101682) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101682_WB_19011506_689.jpg

GTX101682 WB Image

Sample(30 µg whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX101682 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101682_39995_20150522_IP_19011506_749.jpg

GTX101682 IP Image

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody [C2C3], C-term (GTX101682).
Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf