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GRK2 antibody [C2C3], C-term

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682
Anti-GRK2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101682

Cat No. GTX101682

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , Jurkat , NCI-H929

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G protein-coupled receptor kinase 2,ADRBK1,BARK1,BETA-ARK1

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

GRK2 antibody detects GRK2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX101682) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101682

GTX101682 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GRK2(GTX101682) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101682 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101682

GTX101682 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GRK2(GTX101682) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-GRK2 antibody [C2C3], C-term used in Western Blot (WB). GTX101682

GTX101682 WB Image

Sample(30 µg whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX101682 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101682

GTX101682 IP Image

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody [C2C3], C-term (GTX101682).
Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169