Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.
Green: GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min