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GRK2 antibody [HL1562]

Recombinant antibody KO/KD Overexpression
Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038
Anti-GRK2 antibody [HL1562] used in IHC (Paraffin sections) (IHC-P). GTX637038
Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038
Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038
Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038
Anti-GRK2 antibody [HL1562] used in IHC (Paraffin sections) (IHC-P). GTX637038
Anti-GRK2 antibody [HL1562] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637038

Cat. No. GTX637038

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1562

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038

GTX637038 WB Image

KO/KD

Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [HL1562] used in IHC (Paraffin sections) (IHC-P). GTX637038

GTX637038 IHC-P Image

GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038

GTX637038 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038

GTX637038 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [HL1562] used in Western Blot (WB). GTX637038

GTX637038 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (GTX637038) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody [HL1562] used in IHC (Paraffin sections) (IHC-P). GTX637038

GTX637038 IHC-P Image

GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 antibody [HL1562] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637038

GTX637038 ICC/IF Image

GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.
Green: GRK2 stained by GRK2 antibody [HL1562] (GTX637038) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations