GeneTex
United States (US)

GRK2 antibody [N3C3-2]

Overexpression
GTX107915_40009_IHC_M_19011507_511.jpg
GTX107915_40009_20180831_WB_B_19011507_742.jpg
GTX107915_WB_19011507_774.jpg

Cat No. GTX107915

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

H1299 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G Protein-Coupled Receptor Kinase 2,Adrbk1,Bark1,Beta-Ark1,Grk2

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107915_40009_IHC_M_19011507_511.jpg

GTX107915 IHC-P Image

GRK2 antibody [N3C3-2] detects GRK2 protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GRK2 antibody [N3C3-2] (GTX107915) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107915_40009_20180831_WB_B_19011507_742.jpg

GTX107915 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [N3C3-2] (GTX107915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107915_WB_19011507_774.jpg

GTX107915 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107915 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169