GeneTex
United States (US)

GRK2 antibody

KO/KD Overexpression
GTX107601_39981_20180316_WB_KO_watermark_19011506_388.jpg
GTX107601_39981_20180831_WB_B_19011506_233.jpg
GTX107601_39981_IFA_19011506_727.jpg
GTX107601_WB_19011506_437.jpg
GTX107601_39981_IHC_19011506_495.jpg

Cat No. GTX107601

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G Protein-Coupled Receptor Kinase 2,Adrbk1,Bark1,Beta-Ark1,Grk2

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107601_39981_20180316_WB_KO_watermark_19011506_388.jpg

GTX107601 WB Image

KO/KD

Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX107601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX107601_39981_20180831_WB_B_19011506_233.jpg

GTX107601 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX107601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107601_39981_IFA_19011506_727.jpg

GTX107601 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GRK2(GTX107601) antibody at 1:500 dilution.

GTX107601_WB_19011506_437.jpg

GTX107601 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX107601 diluted at 1:1000

GTX107601_39981_IHC_19011506_495.jpg

GTX107601 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GRK2(GTX107601) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169