GeneTex
  • Country / Location Selection

United States (US)

GRK2 antibody

Anti-GRK2 antibody used in Western Blot (WB). GTX130012
Anti-GRK2 antibody used in Western Blot (WB). GTX130012
Anti-GRK2 antibody used in Immunoprecipitation (IP). GTX130012
Anti-GRK2 antibody used in Western Blot (WB). GTX130012

Cat No. GTX130012

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

GRK2 antibody detects GRK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at a dilution of 1:1000.

Anti-GRK2 antibody used in Immunoprecipitation (IP). GTX130012

GTX130012 IP Image

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody (GTX130012).
Western blot analysis was performed using GRK2 antibody (GTX130012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for GRK2 antibody (GTX130012). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GRK2 antibody (GTX130012). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169