GeneTex
United States (US)

GRO gamma antibody

Cat No. GTX10367

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/ml
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Specificity/Sensitivity

The antibody recognizes recombinant rat CINC-2β. It exhibits ~100% cross-reactivity with recombinant rat CINC-2α, 50% cross-reactivity with mouse MIP-2, 35% cross-reactivity with mouse KC, 15% cross-reactivity with rat CINC-1, and 10% cross-reactivity with recombinant human IL-8, GROα, GROβ, and GROγ.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

recombinant rat CINC-2β, expressed in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chemokine (C-X-C Motif) Ligand 3,Cinc-2,Cinc2,Gm1960,Cxcl3

Background

Rat CINC2 beta (Cytokine Induced Neutrophil Chemoattractant 2 beta) is a member of the CXC subfamily of chemokines which also includes three other rat CXC chemokines (CINC1, CINC2 alpha, CINC3/MIP2). The rat chemokines CINC show sequence similarity to human GROs and mouse MIP2 but not IL8. Mature CINC2 beta shares 63% amino acid sequence identity with CINC1 and 80% identity to CINC3. Rat CINC2 beta cDNA encodes a 68 amino acid residue protein. The mature recombinant protein has a predicted molecular mass of approximately 7.6 kDa. Based on sequence homology, it has been suggested that CINCs are the rat counterpart of the human GROs. CINC2 alpha and CINC2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue CINC2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or lipopolysaccharide (LPS) induced inflammatory exudate, though CINC1 and CINC3 are also present. Other cell types known to produce CINC2 proteins include activated macrophages and fibroblasts. Rat CINCs, potent neutrophil attractants and activators, have an important role in the infiltration of neutrophils into inflammatory sites in rats. Recombinant and natural CINC2 proteins are specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC3 specific receptor as well as a second common receptor shared by all CINCs.

Database

SDS
PBS.pdf
Package List Price ($)
$ 429