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GRP1 antibody [N3C3]

KO/KD
Anti-GRP1 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX115211
Anti-GRP1 antibody [N3C3] used in Western Blot (WB). GTX115211
Anti-GRP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115211

Cat. No. GTX115211

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($419),
25 μl ($169)
See all GRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621476

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytohesin 3 , ARNO3 , GRP1 , PSCD3 , cytohesin-3

Background

This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRP1 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX115211

GTX115211 IHC-Wm Image

GRP1 antibody [N3C3] detects GRP1 protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
GRP1 antibody [N3C3] (GTX115211) dilution: 1:200.
Anti-GRP1 antibody [N3C3] used in Western Blot (WB). GTX115211

GTX115211 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX115211 diluted at 1:1000

Anti-GRP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115211

GTX115211 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using GRP1(GTX115211) antibody at 1:200 dilution.

REFERENCE

Application Reference

Clancy JW et al. Nat Cell Biol 2019; (Epub) An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169