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GRP94 antibody [2H3]

Anti-GRP94 antibody [2H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50064
Anti-GRP94 antibody [2H3] used in Western Blot (WB). GTX50064

Cat No. GTX50064

Host

Mouse

Clonality

Monoclonal

Clone Name

2H3

Isotype

IgG2a

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($379)

APPLICATION

Application Note

We recommend the following starting dilutions:
Immunofluorescence: Use at 1:100 ~ 1:200.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TRA1 (676-803aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Heat Shock Protein 90 Beta Family Member 1 , Ecgp , Gp96 , Grp94 , Hel-S-125M , Hel35 , Tra1 , Hsp90B1

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

Tumor rejection antigen 1(TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody [2H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50064

GTX50064 ICC/IF Image

Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)

Anti-GRP94 antibody [2H3] used in Western Blot (WB). GTX50064

GTX50064 WB Image

The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf