APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
| 1:250 |
| 1:200 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Chicken
Immunogen
Chick oviduct GRP94.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1
Background
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Database
Research Area
DATA IMAGES
![Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791](/templates/default/js/lightbox/images/loading.gif "Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791")
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GTX22791 ICC/IF Image
ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX22791 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX22791 IHC-P Image
IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC
Dilution : 1:100
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GTX22791 WB Image
WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10].
Dilution : 1:1000
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GTX22791 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for at least 1 hour at room temperature
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GTX22791 IHC-P Image
IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX22791 WB Image
WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10].
Dilution : 1:200
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GTX22791 IHC-P Image
IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
|
REFERENCE
There are currently no references for GRP94 antibody [9G10] (GTX22791). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GRP94 antibody [9G10] (GTX22791). Be the first to share your experience with this product.
![Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_452_ICC-IF_w_23060620_104.webp "Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1117_IHC-P_w_23060620_387.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1119_IHC-P_w_23060620_840.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1595_WB_w_23060620_858.webp "Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791")
![Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_451_ICC-IF_w_23060620_964.webp "Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1116_IHC-P_w_23060620_550.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1596_WB_w_23060620_938.webp "Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/small/GTX22791/GTX22791_1118_IHC-P_w_23060620_401.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_452_ICC-IF_w_23060620_104.webp "Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1117_IHC-P_w_23060620_387.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1119_IHC-P_w_23060620_840.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1595_WB_w_23060620_858.webp "Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791")
![Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_451_ICC-IF_w_23060620_964.webp "Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1116_IHC-P_w_23060620_550.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")
![Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1596_WB_w_23060620_938.webp "Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791")
![Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791](/upload/website/prouct_img/normal/GTX22791/GTX22791_1118_IHC-P_w_23060620_401.webp "Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791")