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GRP94 antibody [9G10]

Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791
Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791
Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791
Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791
Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791
Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791
Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791
Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791

Cat No. GTX22791

Host

Rat

Clonality

Monoclonal

Clone Name

9G10

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IP, IHC

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Chicken, Pig, Xenopus
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 1:250
IHC-P 1:200
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

Chick oviduct GRP94.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791

GTX22791 IHC-P Image

IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791

GTX22791 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791

GTX22791 IHC-P Image

IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-GRP94 antibody [9G10] used in IHC (Paraffin sections) (IHC-P). GTX22791

GTX22791 IHC-P Image

IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC
Dilution : 1:100

Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791

GTX22791 WB Image

WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10].
Dilution : 1:1000

Anti-GRP94 antibody [9G10] used in Western Blot (WB). GTX22791

GTX22791 WB Image

WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10].
Dilution : 1:200

Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791

GTX22791 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-GRP94 antibody [9G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22791

GTX22791 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for at least 1 hour at room temperature

REFERENCE

There are currently no references for GRP94 antibody [9G10] (GTX22791). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GRP94 antibody [9G10] (GTX22791). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf