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GRP94 antibody [AT94B9]

Anti-GRP94 antibody [AT94B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31955
Anti-GRP94 antibody [AT94B9] used in Western Blot (WB). GTX31955

Cat No. GTX31955

Host Mouse
Clonality Monoclonal
Clone Name AT94B9
Isotype IgG2b
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

WB: 1:3000. ICC/IF: 1:100

Calculated MW

92 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GRP94 (22-803aa) purified from E.coli.

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein 90 Beta Family Member 1,Ecgp,Gp96,Grp94,Hel-S-125M,Hel35,Tra1,Hsp90B1

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES
Anti-GRP94 antibody [AT94B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31955

GTX31955 ICC/IF Image

ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Anti-GRP94 antibody [AT94B9] used in Western Blot (WB). GTX31955

GTX31955 WB Image

The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GRP94 Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: HepG2 cell lysate