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GRP94 antibody [HSP90B1/1192]

Anti-GRP94 antibody [HSP90B1/1192] used in IHC (Paraffin sections) (IHC-P). GTX34773

Cat. No. GTX34773

Host

 Rat

Clonality

 Monoclonal

Clone Name

 HSP90B1/1192

Isotype

 IgG2a

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all GRP94 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human HSP90B1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ECGP , GP96 , GRP94 , HEL35 , HELS125m , HSP90B1 , TRA1 , heat shock protein 90 beta family member 1 , gp96 , ERp99 , Endoplasmin

Cellular Localization

Endoplasmic reticulum lumen,Melanosome

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody [HSP90B1/1192] used in IHC (Paraffin sections) (IHC-P). GTX34773

GTX34773 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34773 GRP94 antibody [HSP90B1/1192].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529