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GRP94 antibody, Internal

Anti-GRP94 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89299
Anti-GRP94 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89299
Anti-GRP94 antibody, Internal used in Western Blot (WB). GTX89299

Cat. No. GTX89299

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, PLA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GRP94 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 3-5μg/ml
PLA Assay dependent

Note :

IHC-P
Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Paraffin embedded Human Uterus.
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89299-PEP).

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722389

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89299

GTX89299 IHC-P Image

IHC-P analysis of human uterus using GTX89299 GRP94 antibody, Internal.
Antigen retrieval : citrate buffer Ph 6
Dilution : 5μg/ml

Anti-GRP94 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89299

GTX89299 IHC-P Image

IHC-P analysis of human liver using GTX89299 GRP94 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-GRP94 antibody, Internal used in Western Blot (WB). GTX89299

GTX89299 WB Image

WB analysis of NIH-3T3 lysate using GTX89299 GRP94 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Li S et al. J Cell Physiol 2019; (Epub) GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway.
Application : PLA
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399